Careful control of both manual and automated techniques for detecting antibodies is essential. Testing of a weak IgG Anti-D with group O R1r red cells in parallel with routine antiglobulin or enzyme tests will confirm the efficacy or otherwise of the chosen technique.
When used by the recommended techniques, the reagent will cause agglutination (clumping) of red cells carrying Rh D antigen. No agglutination when tested against Rh D positive red cells usually indicates a problem with the anti-human globulin or enzyme test.
Lorne Precise Weak Anti-D Control Reagent is prepared from pools of human serum containing low activity Anti-D. The pool is diluted in inert serum to give a final concentration of 0.10 IU/ml Anti-D. ABO antibodies are not absorbed. The reagent is supplied at the optimal dilution for use with the techniques recommended for the reagent to be controlled.