INFORMATION
Adalimumab is a recombinant protein that has the potential to induce humoral immune responses and lead to anti-drug antibody formation; a major cause of loss of response.
These Anti-drug antibodies bind and neutralise the TNFα inhibitors accelerating their clearance and reduces the efficacy of the drugs. Monitoring the efficacy of TNFα inhibitors may help clinicians schedule a successful individualised treatment strategy for their patients.
Total Anti-Adalimumab Antibody ELISA
TOTAL anti-drug antibody ELISAs enable the detection of anti-drug antibodies in the presence of circulating drug, and so are often called “Drug Tolerant” assays. The benefit of drug-tolerant assays is being able to detect lower titres of Anti-drug antibodies which could become clinically significant in certain circumstances.
Total anti-drug Antibody assays offer a greater sensitivity, unmasking the drug-bound portion of anti-drug antibodies in circulation. TOTAL anti-drug antibody analysis may be advantageous when using combination therapy such as immunosuppressants due to this increased sensitivity.
IDKmonitor® Adalimumab total-Anti-drug Antibody ELISA is a quantitative assay that involves a pre-analytical step to dissociate the anti-drug antibodies of interest from the therapeutic Adalimumab. The separated therapeutic Adalimumab is replaced by a biotinylated version of the drug, forming a the new complex which can be captured and quantified on the streptavidin coated microplate.
FEATURES
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Drug tolerant assay for Adalimumab anti-drug antibodies
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Detects Total anti-Adalimumab antibodies in Serum and Plasma
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Preanalytical step to dissociate therapeutic drug from target anti-drug antibody
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1 calibrator, 2 controls
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Capacity for 45 samples in duplicate
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Automatable on ELISA automates (e.g. Dynex D2)
BENEFITS
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Supports the investigation of primary non response and loss of response to Adalimumab
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Drug tolerant assay detects total anti-Adalimumab antibodies (those free- and bound to- Adalimumab)
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Helps predict long term outcome of disease in patients with sub-therapeutic Adalimumab levels
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Identifies patients with low-titre anti-Adalimumab antibodies (e.g. in the case of concomitant immunosuppressant)
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UK publications and evidence base
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Widely available via reference laboratories in UK and Ireland
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Applicable to Gastroenterology, Rheumatology
