INFORMATION
Adalimumab is a recombinant protein that has the potential to induce humoral immune responses and lead to anti-drug antibody formation; a major cause of loss of response.
These Anti-drug antibodies bind and neutralise the TNFα inhibitors accelerating their clearance and reduces the efficacy of the drugs. Monitoring the efficacy of TNFα inhibitors may help clinicians schedule a successful individualised treatment strategy for their patients.
Free Anti-Adalimumab Antibody ELISA
Free anti-drug antibody ELISAs (“drug sensitive assays”) enable the detection of anti-drug antibodies that are not bound to other proteins or therapeutic drugs only. When the dosage of Adalimumab is intensified it has been shown that anti-drug antibodies are not cleared immediately and can actually be masked by the drug level for long periods. This is an important consideration for cost effectiveness of dosage intensification and risk of treatment reactions.
When the drug level is low (undetectable or subtherapeutic) detecting high levels of free anti-Adalimumab antibodies in patients may indicate poorer long term outcomes and suggest the need to switch drug.
IDKmonitor® Adalimumab Free Anti-drug Antibody ELISA is a qualitative assay that detects human antibodies against Adalimumab in EDTA plasma and serum samples by capturing the anti-Adalimumab antibodies on the microplate which is coated with Adalimumab F(ab)2 fragments.
FEATURES
-
Detects free anti-Adalimumab Antibodies in Serum and Plasma
-
Microplate coated with highly specific Adalimumab F(ab)2 fragments
-
1 calibrator, 2 controls
-
Capacity for 45 samples in duplicate
-
Automatable on ELISA automates (e.g. Dynex D2)
BENEFITS
-
Supports the investigation of primary non response and loss of response to Adalimumab
-
Drug sensitive assay only detects free anti-adalimumab antibodies (those not bound to adalimumab)
-
Helps predict long term outcome of disease in patients with sub-therapeutic adalimumab levels
-
Applicable to Gastroenterology, Rheumatology
