The N antigen is part of the MNSs system and was reported in 1927. Anti-N generally reacts at room temperature and so is rarely implicated in Haemolytic Transfusion Reactions and Haemolytic Disease of the Newborn.
The reagent will cause agglutination (clumping) of test red cells, that carry the N antigen, after centrifugation. No agglutination generally indicates the absence of the N antigen.
Lorne Anti-N Lectin blood grouping reagent is prepared from an extract of Vicia, diluted with a sodium chloride solution containing bovine albumin. The reagent is supplied at optimal dilution for use with tube and gel card techniques.