M antigen is part of the MNS system and was first reported in 1927. M antigen expression on red cells can show dosage. Anti-M has rarely been implicated in Haemolytic Disease of the Newborn or in Haemolytic Transfusion Reactions.
The reagent will cause agglutination (clumping) of test red cells, that carry the M antigen, after centrifugation. No agglutination generally indicates the absence of the M antigen.
Lorne Monoclonal Anti-M blood grouping reagent is prepared from murine IgG antibody Clone #LM110/140 diluted in a sodium chloride solution containing bovine albumin. The reagent is supplied at optimal dilution for use with tube, microplate and gel card techniques.