The Lua and Lub antigens were reported in 1945. The expression of the antigens on the red cells can vary widely from person to person. Anti-Lua is not generally associated with Haemolytic Transfusion Reactions but anti-Lub has been. Both anti-Lua and anti-Lub have been implicated in Haemolytic Disease of Newborn.
The reagents will cause indirect agglutination (clumping) of test red cells, that carry the corresponding Lutheran antigen, in the antiglobulin phase of testing. No agglutination generally indicates the absence of the corresponding Lutheran antigen.
Lorne Polyclonal Anti-Lua blood grouping reagent is prepared from human serum diluted in a sodium chloride solution containing macromolecular potentiators and bovine albumin. Each reagent is supplied at optimal dilution for use with indirect Coombs tube test and gel card techniques.