In 1945, Coombs, Mourant and Race described the use of anti-human globulin serum for detecting red cell-bound non-agglutinating antibodies. In 1957, Dacie et al showed that the antibodies present in antiglobulin sera were directed against certain components of complement. Anti-human globulin reagents detect non-agglutinating antibody molecules as well as molecules of complement attached to red cells following in vivo or in vitro antigen-antibody reactions.

When used by the recommended techniques, the reagents will react with immunoglobulins and/or complement attached to the red cell surface, resulting in agglutination (clumping) of adjacent sensitised cells. Cells not sensitised will not be agglutinated.

Lorne Polyspecific Anti-Human Globulin Elite Clear and Anti-Human Globulin Elite Green reagents contain anti-IgG derived from rabbits with non-specific activity removed by absorption and murine monoclonal IgM anti-C3d, Clone BRIC-8. The antibodies are diluted in a buffered solution containing bovine albumin. Each reagent is supplied at optimal dilution for use with spin tube techniques (DAT & IAT).