The Fya and Fyb antigens were reported in 1950 and 1951 respectively. Anti-Fya and anti-Fyb have both been implicated in immediate and delayed Haemolytic Transfusion Reactions and Haemolytic Disease of the Newborn.

The reagents will cause indirect agglutination (clumping) of test red cells, that carry the corresponding Duffy antigen, in the antiglobulin phase of testing. No agglutination generally indicates the absence of the corresponding Duffy antigen.

Lorne Polyclonal Anti-Fyb blood grouping reagents are prepared from human serum diluted in a sodium chloride solution containing macromolecular potentiators and bovine albumin. Each reagent is supplied at optimal dilution for use with indirect Coombs tube test and gel card techniques.